BAIT

GLMU

Y75_p3438
fused N-acetyl glucosamine-1-phosphate uridyltransferase and glucosamine-1-phosphate acetyl transferase
GO Process (0)
GO Function (0)
GO Component (0)
Escherichia coli (K12/W3110)
PREY

RPLO

Y75_p3875
50S ribosomal protein L15
GO Process (0)
GO Function (0)
GO Component (0)
Escherichia coli (K12/W3110)

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Large-scale identification of protein-protein interaction of Escherichia coli K-12.

Arifuzzaman M, Maeda M, Itoh A, Nishikata K, Takita C, Saito R, Ara T, Nakahigashi K, Huang HC, Hirai A, Tsuzuki K, Nakamura S, Altaf-Ul-Amin M, Oshima T, Baba T, Yamamoto N, Kawamura T, Ioka-Nakamichi T, Kitagawa M, Tomita M, Kanaya S, Wada C, Mori H

Protein-protein interactions play key roles in protein function and the structural organization of a cell. A thorough description of these interactions should facilitate elucidation of cellular activities, targeted-drug design, and whole cell engineering. A large-scale comprehensive pull-down assay was performed using a His-tagged Escherichia coli ORF clone library. Of 4339 bait proteins tested, partners were found for 2667, including 779 ... [more]

Genome Res May. 01, 2006; 16(5);686-91 [Pubmed: 16606699]

Throughput

  • High Throughput

Additional Notes

  • Large-scale affinity capture mass spec was used to identify high confidence protein partners in heterologous protein complexes.

Curated By

  • BioGRID