BAIT

MBL-1

CELE_K02H8.1
Protein MBL-1
GO Process (0)
GO Function (0)
GO Component (1)

Gene Ontology Cellular Component

Caenorhabditis elegans
PREY

FUST-1

CELE_C27H5.3, C27H5.3
FUS/TLS RNA binding protein homolog
GO Process (1)
GO Function (0)
GO Component (0)

Gene Ontology Biological Process

Caenorhabditis elegans

Synthetic Growth Defect

A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.

Publication

CRISPR-mediated genetic interaction profiling identifies RNA binding proteins controlling metazoan fitness.

Norris AD, Gracida X, Calarco JA

Genetic interaction screens have aided our understanding of complex genetic traits, diseases, and biological pathways. However, approaches for synthetic genetic analysis with null-alleles in metazoans have not been feasible. Here, we present a CRISPR/Cas9-based Synthetic Genetic Interaction (CRISPR-SGI) approach enabling systematic double-mutant generation. Applying this technique in Caenorhabditis elegans, we comprehensively screened interactions within a set of 14 conserved RNA ... [more]

Elife Jul. 18, 2017; 6(); [Pubmed: 28718764]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: population fitness variant (WBPHENOTYPE:0002118)

Additional Notes

  • CRISPR method
  • CRISPR-SGI screen

Curated By

  • BioGRID