PREY

NPHS2

nphs2l, si:dkey-171o17.6, DKEY-171O17.6
nephrosis 2, idiopathic, steroid-resistant (podocin)
GO Process (3)
GO Function (1)
GO Component (0)

Gene Ontology Molecular Function

Danio rerio

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Lmx1b and FoxC combinatorially regulate podocin expression in podocytes.

He B, Ebarasi L, Zhao Z, Guo J, Ojala JR, Hultenby K, De Val S, Betsholtz C, Tryggvason K

Podocin is a key protein of the kidney podocyte slit diaphragm protein complex, an important part of the glomerular filtration barrier. Mutations in the human podocin gene NPHS2 cause familial or sporadic forms of renal disease owing to the disruption of filtration barrier integrity. The exclusive expression of NPHS2 in podocytes reflects its unique function and raises interesting questions about ... [more]

J Am Soc Nephrol Dec. 01, 2014; 25(12);2764-77 [Pubmed: 24854274]

Throughput

  • Low Throughput

Additional Notes

  • EMSA

Curated By

  • BioGRID