IQGAP1
Gene Ontology Biological Process
- cellular response to calcium ion [IDA]
- cellular response to epidermal growth factor stimulus [IMP]
- energy reserve metabolic process [TAS]
- epidermal growth factor receptor signaling pathway [IMP]
- glomerular visceral epithelial cell development [ISS]
- negative regulation of catalytic activity [TAS]
- neuron projection extension [IMP]
- positive regulation of GTPase activity [TAS]
- positive regulation of protein kinase activity [IMP]
- positive regulation of protein serine/threonine kinase activity [IDA]
- regulation of insulin secretion [TAS]
- signal transduction [TAS]
- small molecule metabolic process [TAS]
Gene Ontology Molecular Function- GTPase activator activity [TAS]
- GTPase inhibitor activity [TAS]
- calcium ion binding [TAS]
- calmodulin binding [IPI]
- phosphatidylinositol-3,4,5-trisphosphate binding [IDA]
- protein binding [IPI]
- protein kinase binding [IPI]
- protein phosphatase binding [IPI]
- protein serine/threonine kinase activator activity [IDA]
- GTPase activator activity [TAS]
- GTPase inhibitor activity [TAS]
- calcium ion binding [TAS]
- calmodulin binding [IPI]
- phosphatidylinositol-3,4,5-trisphosphate binding [IDA]
- protein binding [IPI]
- protein kinase binding [IPI]
- protein phosphatase binding [IPI]
- protein serine/threonine kinase activator activity [IDA]
Gene Ontology Cellular Component
- actin cytoskeleton [ISS]
- actin filament [TAS]
- axon [ISS]
- cell junction [IDA]
- cytoplasm [IDA]
- cytoplasmic ribonucleoprotein granule [IDA]
- extracellular vesicular exosome [IDA]
- extrinsic component of cytoplasmic side of plasma membrane [IDA]
- focal adhesion [IDA]
- growth cone [ISS]
- microtubule [IDA]
- microtubule cytoskeleton [ISS]
- midbody [IDA]
- neuron projection [ISS]
- nucleoplasm [IDA]
- plasma membrane [IDA, TAS]
- slit diaphragm [ISS]
VCP
Gene Ontology Biological Process
- DNA repair [NAS]
- ER-associated ubiquitin-dependent protein catabolic process [IDA, IMP, TAS]
- activation of cysteine-type endopeptidase activity involved in apoptotic process [ISS]
- cellular response to DNA damage stimulus [IDA]
- double-strand break repair [IDA]
- endoplasmic reticulum unfolded protein response [TAS]
- establishment of protein localization [TAS]
- positive regulation of Lys63-specific deubiquitinase activity [IDA]
- positive regulation of proteasomal ubiquitin-dependent protein catabolic process [IDA]
- positive regulation of protein K63-linked deubiquitination [IDA]
- positive regulation of protein catabolic process [IDA]
- positive regulation of protein complex assembly [IDA]
- proteasome-mediated ubiquitin-dependent protein catabolic process [NAS]
- protein N-linked glycosylation via asparagine [IMP]
- protein ubiquitination [IDA, NAS]
- regulation of apoptotic process [TAS]
- retrograde protein transport, ER to cytosol [IDA]
- translesion synthesis [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- Hrd1p ubiquitin ligase complex [IDA]
- cytoplasm [IDA]
- cytosol [IDA]
- endoplasmic reticulum [IDA]
- endoplasmic reticulum membrane [IDA]
- extracellular vesicular exosome [IDA]
- intracellular membrane-bounded organelle [ISS]
- lipid particle [IDA]
- nucleoplasm [IDA]
- nucleus [IDA, TAS]
- perinuclear region of cytoplasm [IDA]
- proteasome complex [IDA]
- site of double-strand break [IDA]
Cross-Linking-MS (XL-MS)
An interaction is detected between two proteins using chemically reactive or photo-activatable cross-linking reagents that covalently link amino acids in close proximity, followed by mass spectrometry analysis to identify the linked peptides (reviewed in PMID 37406423, 37104977). Experiments may be carried with live cells or cell lysates in which all proteins are expressed at endogenous levels (e.g. PMID 34349018, 35235311) or with recombinant proteins (e.g., PMID 28537071).
Publication
Protein interaction landscapes revealed by advanced in vivo cross-linking-mass spectrometry.
Defining protein-protein interactions (PPIs) in their native environment is crucial to understanding protein structure and function. Cross-linking-mass spectrometry (XL-MS) has proven effective in capturing PPIs in living cells; however, the proteome coverage remains limited. Here, we have developed a robust in vivo XL-MS platform to facilitate in-depth PPI mapping by integrating a multifunctional MS-cleavable cross-linker with sample preparation strategies and ... [more]
Throughput
- High Throughput
Additional Notes
- In vivo cross-linking-mass spectrometry (XL-MS) was carried out in HEK-293 cells using the cross-linking reagent Alkyne-A-DSBSO (Azide/Alkyne-tagged, acid-cleavable disuccinimidyl bissulfoxide). High confidence protein interactions were identified based on cross-linked peptides having an FDR < 1%.
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| VCP IQGAP1 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | - | |
| IQGAP1 VCP | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| VCP IQGAP1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| VCP IQGAP1 | Co-localization Co-localization Interaction inferred from two proteins that co-localize in the cell by indirect immunofluorescence only when in addition, if one gene is deleted, the other protein becomes mis-localized. Also includes co-dependent association of proteins with promoter DNA in chromatin immunoprecipitation experiments. | Low | - | BioGRID | - |
Curated By
- BioGRID