NSUN5
TPR
Gene Ontology Biological Process
- MAPK import into nucleus [IMP]
- RNA export from nucleus [IMP]
- RNA import into nucleus [IDA]
- carbohydrate metabolic process [TAS]
- cellular response to heat [IDA]
- cellular response to interferon-alpha [ISS]
- cytokine-mediated signaling pathway [TAS]
- glucose transport [TAS]
- hexose transport [TAS]
- mRNA export from nucleus in response to heat stress [IDA]
- mitotic cell cycle [TAS]
- mitotic nuclear envelope disassembly [TAS]
- mitotic spindle assembly checkpoint [IMP]
- negative regulation of RNA export from nucleus [IDA, IMP]
- negative regulation of transcription from RNA polymerase II promoter [IMP]
- negative regulation of translational initiation [IMP]
- nuclear matrix organization [IMP]
- nuclear pore complex assembly [IMP]
- nuclear pore organization [IMP]
- positive regulation of heterochromatin assembly [IMP]
- positive regulation of intracellular protein transport [IMP]
- positive regulation of mitotic cell cycle spindle assembly checkpoint [IMP]
- positive regulation of protein export from nucleus [ISS]
- positive regulation of protein import into nucleus [IMP]
- protein export from nucleus [IMP]
- protein import into nucleus [IDA, IMP]
- regulation of glucose transport [TAS]
- regulation of mRNA export from nucleus [IMP]
- regulation of mitotic sister chromatid separation [IMP]
- regulation of protein export from nucleus [IMP]
- regulation of protein import into nucleus [IMP]
- regulation of protein stability [IMP]
- regulation of spindle assembly involved in mitosis [IMP]
- response to epidermal growth factor [IDA]
- small molecule metabolic process [TAS]
- transmembrane transport [TAS]
- viral process [TAS]
Gene Ontology Molecular Function- chromatin binding [IDA]
- dynein complex binding [IDA]
- heat shock protein binding [IDA]
- mRNA binding [IDA]
- mitogen-activated protein kinase binding [IDA]
- nucleocytoplasmic transporter activity [IDA]
- poly(A) RNA binding [IDA]
- protein anchor [IMP]
- protein binding [IPI]
- protein homodimerization activity [IDA]
- transporter activity [IMP]
- tubulin binding [IDA]
- chromatin binding [IDA]
- dynein complex binding [IDA]
- heat shock protein binding [IDA]
- mRNA binding [IDA]
- mitogen-activated protein kinase binding [IDA]
- nucleocytoplasmic transporter activity [IDA]
- poly(A) RNA binding [IDA]
- protein anchor [IMP]
- protein binding [IPI]
- protein homodimerization activity [IDA]
- transporter activity [IMP]
- tubulin binding [IDA]
Gene Ontology Cellular Component
Cross-Linking-MS (XL-MS)
An interaction is detected between two proteins using chemically reactive or photo-activatable cross-linking reagents that covalently link amino acids in close proximity, followed by mass spectrometry analysis to identify the linked peptides (reviewed in PMID 37406423, 37104977). Experiments may be carried with live cells or cell lysates in which all proteins are expressed at endogenous levels (e.g. PMID 34349018, 35235311) or with recombinant proteins (e.g., PMID 28537071).
Publication
Two-Dimensional Fractionation Method for Proteome-Wide Cross-Linking Mass Spectrometry Analysis.
Cross-linking mass spectrometry (XL-MS) is an emergent technology for studying protein-protein interactions (PPIs) and elucidating architectures of protein complexes. The development of various MS-cleavable cross-linkers has facilitated the identification of cross-linked peptides, enabling XL-MS studies at the systems level. However, the scope and depth of cellular networks revealed by current XL-MS technologies remain limited. Due to the inherently broad dynamic ... [more]
Throughput
- High Throughput
Additional Notes
- In vitro cross-linking-mass spectrometry (XL-MS) was carried out using HEK-293 cell lysates and the cross-linking reagent DSSO (disuccinimidyl sulfoxide). High confidence protein interactions were identified based on cross-linked peptides having an FDR =< 0.3%.
Curated By
- BioGRID