BAIT

LEASH

CG32471, CG4674, Dmel\CG4674, Dmel_CG4674
CG4674 gene product from transcript CG4674-RA
GO Process (1)
GO Function (0)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Drosophila melanogaster
PREY

PFRX

CG3400, Dmel\CG3400, PFK2, Dmel_CG3400
6-phosphofructo-2-kinase
GO Process (2)
GO Function (2)
GO Component (0)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Drosophila melanogaster

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Comparative interactome analysis of ?-arrestin families in human and Drosophila.

Lee KT, Pranoto IKA, Kim SY, Choi HJ, To NB, Chae H, Lee JY, Kim JE, Kwon YV, Nam JW

The ?-arrestins form a large family of evolutionally conserved modulators that control diverse signaling pathways, including both G-protein-coupled receptor (GPCR)-mediated and non-GPCR-mediated pathways, across eukaryotes. However, unlike ?-arrestins, only a few ?-arrestin targets and functions have been characterized. Here, using affinity purification and mass spectrometry, we constructed interactomes for 6 human and 12 Drosophila ?-arrestins. The resulting high-confidence interactomes comprised ... [more]

Elife Jan. 25, 2024; 12(); [Pubmed: 38270169]

Throughput

  • High Throughput

Additional Notes

  • Affinity capture MS was carried out to identify high confident protein interactors with a FDR of less than or equal to 0.01 and a minimum spectral count of 6

Curated By

  • BioGRID