BAIT

RIC-3

CG15652, CG30296, CG9349, DmRIC-3, Dmel\CG30296, Dmel_CG30296
CG30296 gene product from transcript CG30296-RE
GO Process (2)
GO Function (1)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Drosophila melanogaster
PREY

HEIX

124/1, BG:DS02740.7, CG5876, Dmel\CG5876, ipa-6d, l(2)35Fc, l(2)k12401, Dmel_CG5876
heixuedian
GO Process (1)
GO Function (0)
GO Component (4)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Drosophila melanogaster

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Next-generation large-scale binary protein interaction network for Drosophila melanogaster.

Tang HW, Spirohn K, Hu Y, Hao T, Kovacs IA, Gao Y, Binari R, Yang-Zhou D, Wan KH, Bader JS, Balcha D, Bian W, Booth BW, Cote AG, de Rouck S, Desbuleux A, Goh KY, Kim DK, Knapp JJ, Lee WX, Lemmens I, Li C, Li M, Li R, Lim HJ, Liu Y, Luck K, Markey D, Pollis C, Rangarajan S, Rodiger J, Schlabach S, Shen Y, Sheykhkarimli D, TeeKing B, Roth FP, Tavernier J, Calderwood MA, Hill DE, Celniker SE, Vidal M, Perrimon N, Mohr SE

Generating reference maps of interactome networks illuminates genetic studies by providing a protein-centric approach to finding new components of existing pathways, complexes, and processes. We apply state-of-the-art methods to identify binary protein-protein interactions (PPIs) for Drosophila melanogaster. Four all-by-all yeast two-hybrid (Y2H) screens of?>?10,000 Drosophila proteins result in the 'FlyBi' dataset of 8723 PPIs among 2939 proteins. Testing subsets of ... [more]

Nat Commun Apr. 15, 2023; 14(1);2162 [Pubmed: 37061542]

Throughput

  • High Throughput

Additional Notes

  • interaction identified by large-scale Y2H screening and validated by pairwise testing

Curated By

  • BioGRID