BAIT

L(2)K01209

CG4798, Dmel\CG4798, l(2)k08901, Dmel_CG4798
lethal (2) k01209
GO Process (0)
GO Function (1)
GO Component (0)

Gene Ontology Molecular Function

Drosophila melanogaster
PREY

CG5537

Dmel\CG5537, anon-EST:Posey275, uprt, Dmel_CG5537
CG5537 gene product from transcript CG5537-RC
GO Process (0)
GO Function (1)
GO Component (0)

Gene Ontology Molecular Function

Drosophila melanogaster

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Next-generation large-scale binary protein interaction network for Drosophila melanogaster.

Tang HW, Spirohn K, Hu Y, Hao T, Kovacs IA, Gao Y, Binari R, Yang-Zhou D, Wan KH, Bader JS, Balcha D, Bian W, Booth BW, Cote AG, de Rouck S, Desbuleux A, Goh KY, Kim DK, Knapp JJ, Lee WX, Lemmens I, Li C, Li M, Li R, Lim HJ, Liu Y, Luck K, Markey D, Pollis C, Rangarajan S, Rodiger J, Schlabach S, Shen Y, Sheykhkarimli D, TeeKing B, Roth FP, Tavernier J, Calderwood MA, Hill DE, Celniker SE, Vidal M, Perrimon N, Mohr SE

Generating reference maps of interactome networks illuminates genetic studies by providing a protein-centric approach to finding new components of existing pathways, complexes, and processes. We apply state-of-the-art methods to identify binary protein-protein interactions (PPIs) for Drosophila melanogaster. Four all-by-all yeast two-hybrid (Y2H) screens of?>?10,000 Drosophila proteins result in the 'FlyBi' dataset of 8723 PPIs among 2939 proteins. Testing subsets of ... [more]

Nat Commun Apr. 15, 2023; 14(1);2162 [Pubmed: 37061542]

Throughput

  • High Throughput

Additional Notes

  • MAmmalian Protein-Protein Interaction Trap (MAPPIT) assay
  • interaction identified by large-scale Y2H screening and validated by pairwise testing

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
L(2)K01209 CG5537
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High-BioGRID
-
L(2)K01209 CG5537
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

High-BioGRID
-

Curated By

  • BioGRID