PREY

PEP

CG6143, Dmel\CG6143, PEP/X4, X4/PEP, p100, Dmel_CG6143
Protein on ecdysone puffs
Drosophila melanogaster

Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

Interactions of drosophila cryptochrome.

Ozcelik G, Koca MS, Sunbul B, Yilmaz-Atay F, Demirhan F, Tiryaki B, Cilenk K, Selvi S, Ozturk N

In this study, we investigate the intricate regulatory mechanisms underlying the circadian clock in Drosophila, focusing on the light-induced conformational changes in the cryptochrome (DmCry). Upon light exposure, DmCry undergoes conformational changes that prompt its binding to Timeless and Jetlag proteins, initiating a cascade crucial for the starting of a new circadian cycle. DmCry is subsequently degraded, contributing to the ... [more]

Photochem Photobiol Feb. 05, 2024; (); [Pubmed: 38314442]

Throughput

  • High Throughput

Additional Notes

  • (APEX2) Proximity Label MS was carried out to identify high confidence protein interactors in light conditions

Curated By

  • BioGRID