An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

In vivo proximity biotin ligation identifies the interactome of Egalitarian, a Dynein cargo adaptor.

Baker FC, Neiswender H, Veeranan-Karmegam R, Gonsalvez GB

Numerous motors of the Kinesin family contribute to plus-end-directed microtubule transport. However, almost all transport towards the minus-end of microtubules involves Dynein. Understanding the mechanism by which Dynein transports this vast diversity of cargo is the focus of intense research. In selected cases, adaptors that link a particular cargo with Dynein have been identified. However, the sheer diversity of cargo ... [more]

Development Dec. 15, 2020; 148(22); [Pubmed: 35020877]

Throughput

High Throughput

Curated By

BioGRID

Interaction Summary

ME31B

Gene Ontology Biological Process

Gene Ontology Molecular Function

ATP-dependent RNA helicase activity [ISS, NAS]RNA binding [IDA]protein binding [IPI]

Gene Ontology Cellular Component

ONECUT

Gene Ontology Biological Process

Gene Ontology Molecular Function

DNA binding [NAS]RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]

Gene Ontology Cellular Component

Proximity Label-MS

Publication

In vivo proximity biotin ligation identifies the interactome of Egalitarian, a Dynein cargo adaptor.

Throughput

Curated By

ATP-dependent RNA helicase activity [ISS, NAS]
RNA binding [IDA]
protein binding [IPI]

DNA binding [NAS]
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]