An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

In vivo proximity biotin ligation identifies the interactome of Egalitarian, a Dynein cargo adaptor.

Baker FC, Neiswender H, Veeranan-Karmegam R, Gonsalvez GB

Numerous motors of the Kinesin family contribute to plus-end-directed microtubule transport. However, almost all transport towards the minus-end of microtubules involves Dynein. Understanding the mechanism by which Dynein transports this vast diversity of cargo is the focus of intense research. In selected cases, adaptors that link a particular cargo with Dynein have been identified. However, the sheer diversity of cargo ... [more]

Development Dec. 15, 2020; 148(22); [Pubmed: 35020877]

Throughput

High Throughput

Curated By

BioGRID

Interaction Summary

ME31B

Gene Ontology Biological Process

Gene Ontology Molecular Function

ATP-dependent RNA helicase activity [ISS, NAS]RNA binding [IDA]protein binding [IPI]

Gene Ontology Cellular Component

COVB