C/EBP? represents a paradigm intrinsically disordered transcription factor containing short linear motifs and post-translational modifications (PTM). Unraveling C/EBP? protein interaction networks is a prerequisite for understanding the multi-modal functions of C/EBP? in hematopoiesis and leukemia. Here, we combined arrayed peptide matrix screening (PRISMA) with BioID to generate an in vivo validated and isoform specific interaction map of C/EBP?. The myeloid C/EBP? ... [more]

iScience Jun. 25, 2021; 24(6);102686 [Pubmed: 34189442]

Throughput

High Throughput

Additional Notes

Used arrayed peptide matrix screening (PRISMA) method
significance threshold (FDR less than 0.1)

Curated By

BioGRID

Interaction Summary

CEBPA

Gene Ontology Biological Process

Gene Ontology Molecular Function

DNA binding [TAS]RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity [NAS]protein binding [IPI]sequence-specific DNA binding transcription factor activity [IDA]transcription factor binding [IPI]transcription regulatory region DNA binding [IDA]

Gene Ontology Cellular Component

CMSS1

Gene Ontology Molecular Function

poly(A) RNA binding [IDA]

Protein-peptide

Publication

PRISMA and BioID disclose a motifs-based interactome of the intrinsically disordered transcription factor C/EBP?.

Throughput

Additional Notes

Curated By

DNA binding [TAS]
RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity [NAS]
protein binding [IPI]
sequence-specific DNA binding transcription factor activity [IDA]
transcription factor binding [IPI]
transcription regulatory region DNA binding [IDA]