PREY

PSMD10

dJ889N15.2, p28, p28(GANK), RP5-889N15.3
proteasome (prosome, macropain) 26S subunit, non-ATPase, 10
GO Process (32)
GO Function (2)
GO Component (8)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Homo sapiens

Protein-peptide

An interaction is detected between a protein and a peptide derived from an interaction partner. This includes phage display experiments.

Publication

PRISMA and BioID disclose a motifs-based interactome of the intrinsically disordered transcription factor C/EBP?.

Ramberger E, Sapozhnikova V, Kowenz-Leutz E, Zimmermann K, Nicot N, Nazarov PV, Perez-Hernandez D, Reimer U, Mertins P, Dittmar G, Leutz A

C/EBP? represents a paradigm intrinsically disordered transcription factor containing short linear motifs and post-translational modifications (PTM). Unraveling C/EBP? protein interaction networks is a prerequisite for understanding the multi-modal functions of C/EBP? in hematopoiesis and leukemia. Here, we combined arrayed peptide matrix screening (PRISMA) with BioID to generate an in vivo validated and isoform specific interaction map of C/EBP?. The myeloid C/EBP? ... [more]

iScience Jun. 25, 2021; 24(6);102686 [Pubmed: 34189442]

Throughput

  • High Throughput

Additional Notes

  • Used arrayed peptide matrix screening (PRISMA) method
  • significance threshold (FDR less than 0.1)

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
PSMD10 CEBPA
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
-

Curated By

  • BioGRID