BAIT
PLK-1
CELE_C14B9.4
Protein PLK-1
GO Process (15)
GO Function (2)
GO Component (5)
Gene Ontology Biological Process
- apoptotic process [IMP]
- centrosome cycle [IMP]
- embryo development ending in birth or egg hatching [IMP]
- female meiosis II [IMP]
- hermaphrodite genitalia development [IMP]
- meiotic chromosome segregation [IMP]
- meiotic nuclear division [IMP]
- mitotic nuclear division [IMP]
- mitotic nuclear envelope disassembly [IMP, TAS]
- mitotic spindle organization [IMP]
- nematode larval development [IMP]
- oogenesis [IMP]
- polar body extrusion after meiotic divisions [IMP]
- regulation of cell cycle [IMP]
- reproduction [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Caenorhabditis elegans
PREY
GLN-3
CELE_Y105C5B.28
Protein GLN-3
GO Process (0)
GO Function (0)
GO Component (0)
Caenorhabditis elegans
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
A modified TurboID approach identifies tissue-specific centriolar components in C. elegans.
Proximity-dependent labeling approaches such as BioID have been a great boon to studies of protein-protein interactions in the context of cytoskeletal structures such as centrosomes which are poorly amenable to traditional biochemical approaches like immunoprecipitation and tandem affinity purification. Yet, these methods have so far not been applied extensively to invertebrate experimental models such as C. elegans given the long ... [more]
PLoS Genet Apr. 01, 2022; 18(4);e1010150 [Pubmed: 35442950]
Throughput
- High Throughput
Additional Notes
- indirect Turbo-ID
Curated By
- BioGRID