PREY

RB1

OSRC, PPP1R130, RB, p105-Rb, pRb, pp110, RP11-174I10.1
retinoblastoma 1
GO Process (24)
GO Function (11)
GO Component (6)
Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Identification and functional characterization of transcriptional activators in human cells.

Alerasool N, Leng H, Lin ZY, Gingras AC, Taipale M

Transcription is orchestrated by thousands of transcription factors (TFs) and chromatin-associated proteins, but how these are causally connected to transcriptional activation is poorly understood. Here, we conduct an unbiased proteome-scale screen to systematically uncover human proteins that activate transcription in a natural chromatin context. By combining interaction proteomics and chemical inhibitors, we delineate the preference of these transcriptional activators for ... [more]

Mol Cell Dec. 03, 2021; 82(3);677-695.e7 [Pubmed: 35016035]

Throughput

  • High Throughput

Additional Notes

  • Affinity-capture MS was carried out to identify high confidence protein-protein interactions with a Bayesian FDR cutoff of less than or equal to 5%

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
CTBP1 RB1
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High0.9991BioGRID
1194097
CTBP1 RB1
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High0.996BioGRID
2237033
CTBP1 RB1
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High0.9979BioGRID
3072206

Curated By

  • BioGRID