An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Identification and functional characterization of transcriptional activators in human cells.

Alerasool N, Leng H, Lin ZY, Gingras AC, Taipale M

Transcription is orchestrated by thousands of transcription factors (TFs) and chromatin-associated proteins, but how these are causally connected to transcriptional activation is poorly understood. Here, we conduct an unbiased proteome-scale screen to systematically uncover human proteins that activate transcription in a natural chromatin context. By combining interaction proteomics and chemical inhibitors, we delineate the preference of these transcriptional activators for ... [more]

Mol Cell Dec. 03, 2021; 82(3);677-695.e7 [Pubmed: 35016035]

Throughput

High Throughput

Additional Notes

Affinity-capture MS was carried out to identify high confidence protein-protein interactions with a Bayesian FDR cutoff of less than or equal to 5%

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
NCKIPSD COPS3	Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.	Havugimana PC (2022)	High	-	BioGRID	3454287

Curated By

BioGRID

Interaction Summary

NCKIPSD

Gene Ontology Biological Process

Gene Ontology Molecular Function

cytoskeletal protein binding [NAS]protein binding [IPI]

Gene Ontology Cellular Component

COPS3