Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

Identification and functional characterization of transcriptional activators in human cells.

Alerasool N, Leng H, Lin ZY, Gingras AC, Taipale M

Transcription is orchestrated by thousands of transcription factors (TFs) and chromatin-associated proteins, but how these are causally connected to transcriptional activation is poorly understood. Here, we conduct an unbiased proteome-scale screen to systematically uncover human proteins that activate transcription in a natural chromatin context. By combining interaction proteomics and chemical inhibitors, we delineate the preference of these transcriptional activators for ... [more]

Mol Cell Dec. 03, 2021; 82(3);677-695.e7 [Pubmed: 35016035]

Throughput

  • High Throughput

Additional Notes

  • Proximity-label MS was carried out to identify high confidence protein-protein interactions with a Bayesian FDR cutoff of less than or equal to 5%

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
SS18L2 ARID1A
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High0.9921BioGRID
3135078

Curated By

  • BioGRID