BAIT

LET-7

CELE_C05G5.6, CELE_C05G5.6.2, CELE_C05G5.6.3, CELE_C05G5.6.1, C05G5.6
let-7 encodes a 21-nucleotide microRNA (also known as a small temporal RNA, or stRNA) that is conserved in bilateral animal species (including vertebrates, ascidians, hemichordates, molluscs, annelids and arthropods), but is not found in cnidarians, poriferans, fungi, plants, protists, archaea, or bacteria; let-7 is required for the transition from late larval to adult cell fates, and for larval viability; like lin-4, let-7 is complementary to sequences in the 3' untranslated regions of target genes that it negatively regulates; let-7 RNA expression is first detected at late larval stages and is negatively regulated by the product of one of its targets, hbl-1, in a negative feedback loop; the temporal regulatory element (TRE), situated about 1200 base pairs upstream of the start of the mature let-7 RNA, is both necessary and sufficient for temporal upregulation of let-7 transcription; DCR-1, ALG-1, and ALG-2 are required for the maturation and activity of let-7 stRNA; since let-7 homologs are expressed in zebrafish embryos and in adult annelids and molluscs, let-7 may have a conserved function in heterochronic developmental regulation in bilateria.
Caenorhabditis elegans
PREY

RNP-7

CELE_K04G7.10, K04G7.10
RNP (RRM RNA binding domain) containing
Caenorhabditis elegans

Protein-RNA

An interaction is detected between and protein and an RNA in vitro.

Publication

Functional identification of microRNA-centered complexes in C. elegans.

Hebbar S, Panzade G, Vashisht AA, Wohlschlegel JA, Veksler-Lublinsky I, Zinovyeva AY

microRNAs (miRNAs) are crucial for normal development and physiology. To identify factors that might coordinate with miRNAs to regulate gene expression, we used 2'O-methylated oligonucleotides to precipitate Caenorhabditis elegans let-7, miR-58, and miR-2 miRNAs and the associated proteins. A total of 211 proteins were identified through mass-spectrometry analysis of miRNA co-precipitates, which included previously identified interactors of key miRNA pathway ... [more]

Sci Rep May. 03, 2022; 12(1);7133 [Pubmed: 35504914]

Throughput

  • High Throughput

Additional Notes

  • miRNA pulldowns were carried out to identify high confidence protein interactors that were greater than or equal to 4-fold enriched over controls, had a minimum NSAF of 4 and were identified in all replicates

Curated By

  • BioGRID