BAIT

MIR-2

CELE_M04C9.7, cel-mir-2, M04C9.7
mir-2 encodes a microRNA, a small non-protein coding RNA; many of the known microRNAs are involved in post-transcriptional regulation of genes; mir-2 is conserved in C. briggsae as well as in Drosophila; miR-2 is expressed at all life stages and most abundantly in the L1 larval stage; the precise function of miR-2 is not know
GO Process (0)
GO Function (0)
GO Component (0)
Caenorhabditis elegans
PREY

PES-4

CELE_Y119D3B.17, Y119D3B.17
pes-4 encodes a protein that contains two type 1 KH domains, RNA-binding domains that are present in a diverse group of nucleic acid-binding proteins; pes-4 was identified in promoter-trapping screens and by homology, is predicted to function as an RNA-binding protein; however, the precise role of PES-4 in C. elegans development and/or behavior is not yet known; a pes-4 reporter is expressed in three rectal epithelial cells from the L1 larval stage through adulthood and is also seen dispersed throughout the pharynx in all postembryonic stages.
GO Process (0)
GO Function (0)
GO Component (0)
Caenorhabditis elegans

Protein-RNA

An interaction is detected between and protein and an RNA in vitro.

Publication

Functional identification of microRNA-centered complexes in C. elegans.

Hebbar S, Panzade G, Vashisht AA, Wohlschlegel JA, Veksler-Lublinsky I, Zinovyeva AY

microRNAs (miRNAs) are crucial for normal development and physiology. To identify factors that might coordinate with miRNAs to regulate gene expression, we used 2'O-methylated oligonucleotides to precipitate Caenorhabditis elegans let-7, miR-58, and miR-2 miRNAs and the associated proteins. A total of 211 proteins were identified through mass-spectrometry analysis of miRNA co-precipitates, which included previously identified interactors of key miRNA pathway ... [more]

Sci Rep May. 03, 2022; 12(1);7133 [Pubmed: 35504914]

Throughput

  • High Throughput

Additional Notes

  • miRNA pulldowns were carried out to identify high confidence protein interactors that were greater than or equal to 4-fold enriched over controls, had a minimum NSAF of 4 and were identified in all replicates

Curated By

  • BioGRID