BAIT
ALP-1
CELE_T11B7.4
Protein ALP-1
GO Process (3)
GO Function (1)
GO Component (1)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Caenorhabditis elegans
PREY
PAT-2
CELE_F54F2.1, vab-20, F54F2.1
pat-2 encodes an alpha integrin subunit; during embryogenesis, pat-2 is essential for body wall muscle assembly and function and hence, proper elongation and hatching.
GO Process (12)
GO Function (1)
GO Component (4)
Gene Ontology Biological Process
- embryo development ending in birth or egg hatching [IMP]
- inductive cell migration [IMP]
- locomotion [IMP]
- nematode larval development [IMP]
- oviposition [IMP]
- positive regulation of engulfment of apoptotic cell [IMP]
- positive regulation of locomotion [IMP]
- protein catabolic process [IMP]
- receptor-mediated endocytosis [IMP]
- reproduction [IMP]
- secretion by cell [IMP]
- striated muscle contraction involved in embryonic body morphogenesis [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Caenorhabditis elegans
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Mass spectrometry-based shotgun proteomic analysis of C. elegans protein complexes.
Mass spectrometry (MS)-based shotgun proteomics is an enabling technology for the study of C. elegans proteins. When coupled with co-immunoprecipitation (CoIP), new interactions and functions among proteins can be discovered. We provide a general background on protein complexes and methods for their analysis, along with the lifecycle and interaction types of proteins that ultimately define the identifiable components of protein ... [more]
WormBook Jun. 24, 2014; ();1-18 [Pubmed: 24967700]
Throughput
- High Throughput
Curated By
- BioGRID