BAIT

PAQR-2

CELE_Y32H12A.5, Y32H12A.5
Progestin and AdipoQ Receptor family
GO Process (2)
GO Function (2)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Caenorhabditis elegans
PREY

IGLR-2

CELE_ZC262.3
Protein IGLR-2
GO Process (0)
GO Function (0)
GO Component (0)
Caenorhabditis elegans

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

AdipoR2 recruits protein interactors to promote fatty acid elongation and membrane fluidity.

Ruiz M, Devkota R, Kaper D, Ruhanen H, Busayavalasa K, Radovic U, Henricsson M, Kaekelae R, Boren J, Pilon M

The human AdipoR2 and its Caenorhabditis elegans homolog PAQR-2 are multipass plasma membrane proteins that protect cells against membrane rigidification. However, how AdipoR2 promotes membrane fluidity mechanistically is not clear. Using 13C-labeled fatty acids, we show that AdipoR2 can promote the elongation and incorporation of membrane-fluidizing polyunsaturated fatty acids into phospholipids. To elucidate the molecular basis of these activities, we ... [more]

J Biol Chem Jun. 01, 2023; 299(6);104799 [Pubmed: 37164154]

Throughput

  • High Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
IGLR-2 PAQR-2
PCA
PCA

A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Low-WormBase
-

Curated By

  • BioGRID