Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
A genome-wide study of PDZ-domain interactions in C. elegans reveals a high frequency of non-canonical binding.
Proteins may evolve through the recruitment and modification of discrete domains, and in many cases, protein action can be dissected at the domain level. PDZ domains are found in many important structural and signaling complexes, and are generally thought to interact with their protein partners through a C-terminal consensus sequence. We undertook a comprehensive search for protein partners of all ... [more]
BMC Genomics Nov. 30, 2010; 11(0);671 [Pubmed: 21110867]
Throughput
- High Throughput
Curated By
- BioGRID