BAIT

PTRFA

si:ch211-209d14.2
polymerase I and transcript release factor a
GO Process (0)
GO Function (0)
GO Component (0)
Danio rerio
PREY

DMD

Dp71, cb664, im:6911785, zfDYS, zgc:110165
dystrophin
Danio rerio

Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

In vivo proteomic mapping through GFP-directed proximity-dependent biotin labelling in zebrafish.

Xiong Z, Lo HP, McMahon KA, Martel N, Jones A, Hill MM, Parton RG, Hall TE

Protein interaction networks are crucial for complex cellular processes. However, the elucidation of protein interactions occurring within highly specialised cells and tissues is challenging. Here, we describe the development, and application, of a new method for proximity-dependent biotin labelling in whole zebrafish. Using a conditionally stabilised GFP-binding nanobody to target a biotin ligase to GFP-labelled proteins of interest, we show ... [more]

Elife Feb. 16, 2021; 10(); [Pubmed: 33591275]

Throughput

  • High Throughput

Curated By

  • BioGRID