Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

Binding of USP4 to cortactin enhances cell migration in HCT116 human colon cancer cells.

Yun SI, Kwak C, Lee SY, Shin S, Oh C, Kim JS, Rhee HW, Kim KK

Ubiquitin-specific protease 4 (USP4) is highly overexpressed in colon cancer and acts as a potent protooncogenic protein by deubiquitinating ?-catenin. However, its prominent roles in tumor formation and migration in cancer cells are not fully understood by its deubiquitinating enzyme (DUB) activity on ?-catenin. Thus, we investigated an additional role of USP4 in cancer. In this study, we identified cortactin ... [more]

FASEB J May. 01, 2023; 37(5);e22900 [Pubmed: 37039823]

Throughput

  • High Throughput

Curated By

  • BioGRID