PREY

ABL2

ABLL, ARG, RP11-177A2.3
ABL proto-oncogene 2, non-receptor tyrosine kinase
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

ABL kinases regulate the stabilization of HIF-1? and MYC through CPSF1.

Mayro B, Hoj JP, Cerda-Smith CG, Hutchinson HM, Caminear MW, Thrash HL, Winter PS, Wardell SE, McDonnell DP, Wu C, Wood KC, Pendergast AM

The hypoxia-inducible factor 1-? (HIF-1?) enables cells to adapt and respond to hypoxia (Hx), and the activity of this transcription factor is regulated by several oncogenic signals and cellular stressors. While the pathways controlling normoxic degradation of HIF-1? are well understood, the mechanisms supporting the sustained stabilization and activity of HIF-1? under Hx are less clear. We report that ABL ... [more]

Proc Natl Acad Sci U S A Apr. 18, 2023; 120(16);e2210418120 [Pubmed: 37040401]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
CUL4A ABL2
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High-BioGRID
-
ABL2 CUL4A
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
-

Curated By

  • BioGRID