An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

PRISMA: Protein Interaction Screen on Peptide Matrix Reveals Interaction Footprints and Modifications- Dependent Interactome of Intrinsically Disordered C/EBP?.

Dittmar G, Hernandez DP, Kowenz-Leutz E, Kirchner M, Kahlert G, Wesolowski R, Baum K, Knoblich M, Hofstaetter M, Muller A, Wolf J, Reimer U, Leutz A

CCAAT enhancer-binding protein beta (C/EBP?) is a pioneer transcription factor that specifies cell differentiation. C/EBP? is intrinsically unstructured, a molecular feature common to many proteins involved in signal processing and epigenetics. The structure of C/EBP? differs depending on alternative translation initiation and multiple post-translational modifications (PTM). Mutation of distinct PTM sites in C/EBP? alters protein interactions and cell differentiation, suggesting ... [more]

iScience Mar. 29, 2019; 13();351-370 [Pubmed: 30884312]

Throughput

High Throughput

Additional Notes

FDR cutoff of 5%
IP-MS in SU-DHL1 cells

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
CEBPB EHMT2	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Pless O (2008)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

CEBPB

Gene Ontology Biological Process

Gene Ontology Molecular Function

DNA binding [TAS]RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity [ISS]protein binding [IPI]sequence-specific DNA binding transcription factor activity [NAS]

Gene Ontology Cellular Component

EHMT2

Gene Ontology Biological Process

Gene Ontology Molecular Function

C2H2 zinc finger domain binding [IPI]histone methyltransferase activity (H3-K27 specific) [ISS]histone methyltransferase activity (H3-K9 specific) [ISS]histone-lysine N-methyltransferase activity [IMP]p53 binding [IPI]protein binding [IPI]protein-lysine N-methyltransferase activity [IDA]