Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

PRISMA: Protein Interaction Screen on Peptide Matrix Reveals Interaction Footprints and Modifications- Dependent Interactome of Intrinsically Disordered C/EBP?.

Dittmar G, Hernandez DP, Kowenz-Leutz E, Kirchner M, Kahlert G, Wesolowski R, Baum K, Knoblich M, Hofstaetter M, Muller A, Wolf J, Reimer U, Leutz A

CCAAT enhancer-binding protein beta (C/EBP?) is a pioneer transcription factor that specifies cell differentiation. C/EBP? is intrinsically unstructured, a molecular feature common to many proteins involved in signal processing and epigenetics. The structure of C/EBP? differs depending on alternative translation initiation and multiple post-translational modifications (PTM). Mutation of distinct PTM sites in C/EBP? alters protein interactions and cell differentiation, suggesting ... [more]

iScience Mar. 29, 2019; 13();351-370 [Pubmed: 30884312]

Throughput

  • High Throughput

Additional Notes

  • FDR cutoff of 5%
  • IP-MS in SU-DHL1 cells

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
CEBPB KLC2
Co-fractionation
Co-fractionation

Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.

High-BioGRID
3439017

Curated By

  • BioGRID