An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

PRISMA: Protein Interaction Screen on Peptide Matrix Reveals Interaction Footprints and Modifications- Dependent Interactome of Intrinsically Disordered C/EBP?.

Dittmar G, Hernandez DP, Kowenz-Leutz E, Kirchner M, Kahlert G, Wesolowski R, Baum K, Knoblich M, Hofstaetter M, Muller A, Wolf J, Reimer U, Leutz A

CCAAT enhancer-binding protein beta (C/EBP?) is a pioneer transcription factor that specifies cell differentiation. C/EBP? is intrinsically unstructured, a molecular feature common to many proteins involved in signal processing and epigenetics. The structure of C/EBP? differs depending on alternative translation initiation and multiple post-translational modifications (PTM). Mutation of distinct PTM sites in C/EBP? alters protein interactions and cell differentiation, suggesting ... [more]

iScience Mar. 29, 2019; 13();351-370 [Pubmed: 30884312]

Throughput

High Throughput

Additional Notes

FDR cutoff of 5%
IP-MS in SU-DHL1 cells

Curated By

BioGRID

Interaction Summary

CEBPB

Gene Ontology Biological Process

Gene Ontology Molecular Function

DNA binding [TAS]RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity [ISS]protein binding [IPI]sequence-specific DNA binding transcription factor activity [NAS]

Gene Ontology Cellular Component

NAIF1