BAIT

PKA-R2

BcDNA:GM01761, CG15862, Cos, Cos-1, Cos1, Dmel\CG15862, Epa, PKA, PKA RII, PKA-RII, RII, RII[[DR]], Dmel_CG15862
cAMP-dependent protein kinase R2
Drosophila melanogaster
PREY

TAF11

CG4079, Dmel\CG4079, TAF, TAF1, TAF30b, TAF30beta, TAF[II]30beta, TAF[[II]], TAF[[II]]30beta, TFIID, TFIID 28 beta, TFIID-p28beta, d30beta, dTAFII30, dTAFII30 beta, dTAFII30beta, dTAF[[II]]30, dTAF[[II]]30beta, Dmel_CG4079
TBP-associated factor 11
Drosophila melanogaster

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Analysis of the expression patterns, subcellular localisations and interaction partners of Drosophila proteins using a pigP protein trap library.

Lowe N, Rees JS, Roote J, Ryder E, Armean IM, Johnson G, Drummond E, Spriggs H, Drummond J, Magbanua JP, Naylor H, Sanson B, Bastock R, Huelsmann S, Trovisco V, Landgraf M, Knowles-Barley S, Armstrong JD, White-Cooper H, Hansen C, Phillips RG, , Lilley KS, Russell S, St Johnston D

Although we now have a wealth of information on the transcription patterns of all the genes in the Drosophila genome, much less is known about the properties of the encoded proteins. To provide information on the expression patterns and subcellular localisations of many proteins in parallel, we have performed a large-scale protein trap screen using a hybrid piggyBac vector carrying ... [more]

Development Oct. 01, 2014; 141(20);3994-4005 [Pubmed: 25294943]

Throughput

  • High Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
PKA-R2 TAF11
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

High-BioGRID
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Curated By

  • BioGRID