BAIT

ATPSYN-BETA

ATP, ATP syn-beta, ATP-syn-B, ATP-synbeta, ATPB, ATPase beta, ATPasebeta, ATPsyn b, ATPsyn-&bgr, ATPsyn-b, CG11154, Dmel\CG11154, beta-ATPase, Dmel_CG11154
ATP synthase-beta
GO Process (2)
GO Function (1)
GO Component (3)
Drosophila melanogaster
PREY

CG10340

Dmel\CG10340, anon-EST:fe2A5, anon2A5, Dmel_CG10340
CG10340 gene product from transcript CG10340-RA
GO Process (0)
GO Function (0)
GO Component (0)
Drosophila melanogaster

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Analysis of the expression patterns, subcellular localisations and interaction partners of Drosophila proteins using a pigP protein trap library.

Lowe N, Rees JS, Roote J, Ryder E, Armean IM, Johnson G, Drummond E, Spriggs H, Drummond J, Magbanua JP, Naylor H, Sanson B, Bastock R, Huelsmann S, Trovisco V, Landgraf M, Knowles-Barley S, Armstrong JD, White-Cooper H, Hansen C, Phillips RG, , Lilley KS, Russell S, St Johnston D

Although we now have a wealth of information on the transcription patterns of all the genes in the Drosophila genome, much less is known about the properties of the encoded proteins. To provide information on the expression patterns and subcellular localisations of many proteins in parallel, we have performed a large-scale protein trap screen using a hybrid piggyBac vector carrying ... [more]

Development Oct. 01, 2014; 141(20);3994-4005 [Pubmed: 25294943]

Throughput

  • High Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
CG10340 ATPSYN-BETA
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

High-BioGRID
-

Curated By

  • BioGRID