An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

Genome-wide CRISPR screens identify novel regulators of wild-type and mutant p53 stability.

Lue Y, Cho T, Mukherjee S, Suarez CF, Gonzalez-Foutel NS, Malik A, Martinez S, Dervovic D, Oh RH, Langille E, Al-Zahrani KN, Hoeg L, Lin ZY, Tsai R, Mbamalu G, Rotter V, Ashton-Prolla P, Moffat J, Chemes LB, Gingras AC, Oren M, Durocher D, Schramek D

Tumor suppressor p53 (TP53) is frequently mutated in cancer, often resulting not only in loss of its tumor-suppressive function but also acquisition of dominant-negative and even oncogenic gain-of-function traits. While wild-type p53 levels are tightly regulated, mutants are typically stabilized in tumors, which is crucial for their oncogenic properties. Here, we systematically profiled the factors that regulate protein stability of ... [more]

Mol Syst Biol Jun. 01, 2024; 20(6);719-740 [Pubmed: 38580884]

Throughput

High Throughput

Additional Notes

BioID
High confidence BioID interactions had a minimum unique mass spectral count of 2 and SAINT BFDR score =< 0.01
MG132 proteasome inhibitor treatment

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
CCDC6 LRSAM1	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Luck K (2020)	High	-	BioGRID	2712289

Curated By

BioGRID

Interaction Summary

CCDC6

Gene Ontology Molecular Function

protein binding [IPI]structural constituent of cytoskeleton [TAS]

Gene Ontology Cellular Component

LRSAM1

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]ubiquitin-protein transferase activity [IDA]