Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

Genome-wide CRISPR screens identify novel regulators of wild-type and mutant p53 stability.

Lue Y, Cho T, Mukherjee S, Suarez CF, Gonzalez-Foutel NS, Malik A, Martinez S, Dervovic D, Oh RH, Langille E, Al-Zahrani KN, Hoeg L, Lin ZY, Tsai R, Mbamalu G, Rotter V, Ashton-Prolla P, Moffat J, Chemes LB, Gingras AC, Oren M, Durocher D, Schramek D

Tumor suppressor p53 (TP53) is frequently mutated in cancer, often resulting not only in loss of its tumor-suppressive function but also acquisition of dominant-negative and even oncogenic gain-of-function traits. While wild-type p53 levels are tightly regulated, mutants are typically stabilized in tumors, which is crucial for their oncogenic properties. Here, we systematically profiled the factors that regulate protein stability of ... [more]

Mol Syst Biol Jun. 01, 2024; 20(6);719-740 [Pubmed: 38580884]

Throughput

  • High Throughput

Additional Notes

  • BioID
  • High confidence BioID interactions had a minimum unique mass spectral count of 2 and SAINT BFDR score =< 0.01
  • MG132 proteasome inhibitor treatment

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
FBXO42 ATP1A1
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High-BioGRID
-

Curated By

  • BioGRID