WWP2
Gene Ontology Biological Process
- cellular protein modification process [TAS]
- negative regulation of gene expression [IMP]
- negative regulation of protein transport [IMP]
- negative regulation of sequence-specific DNA binding transcription factor activity [ISS]
- negative regulation of transcription from RNA polymerase II promoter [IMP, ISS]
- negative regulation of transcription, DNA-templated [ISS]
- negative regulation of transporter activity [IDA]
- proteasome-mediated ubiquitin-dependent protein catabolic process [IMP]
- protein K63-linked ubiquitination [ISS]
- protein autoubiquitination [IDA]
- protein ubiquitination [IDA]
- protein ubiquitination involved in ubiquitin-dependent protein catabolic process [IBA]
- regulation of ion transmembrane transport [IDA]
- regulation of membrane potential [IDA]
- regulation of potassium ion transmembrane transporter activity [IDA]
- viral entry into host cell [TAS]
Gene Ontology Molecular Function
PDCD6IP
Gene Ontology Biological Process
- positive regulation of exosomal secretion [IMP]
- positive regulation of extracellular vesicular exosome assembly [IMP]
- regulation of extracellular vesicular exosome assembly [IMP]
- ubiquitin-independent protein catabolic process via the multivesicular body sorting pathway [IMP]
- viral budding via host ESCRT complex [IGI]
- viral life cycle [TAS]
- viral process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Biochemical Activity (Ubiquitination)
An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation.
Publication
Avidity-Based Method for the Efficient Generation of Monoubiquitinated Recombinant Proteins.
Monoubiquitination of proteins governs diverse physiological processes, and its dysregulation is implicated in multiple pathologies. The difficulty of preparing sufficient material often complicates the biophysical studies of monoubiquitinated recombinant proteins. Here we describe a robust avidity-based method that overcomes this problem. As a proof-of-concept, we produced milligram quantities of two monoubiquitinated targets, Parkinson's protein ?-synuclein and ESCRT-protein ALIX, using NEDD4-family ... [more]
Throughput
- Low Throughput
Additional Notes
- E2: UBE2D3
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| WWP2 PDCD6IP | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | 1515042 |
Curated By
- BioGRID