GLS
Gene Ontology Biological Process
- cellular amino acid biosynthetic process [TAS]
- cellular nitrogen compound metabolic process [TAS]
- glutamate biosynthetic process [IDA, TAS]
- glutamate secretion [TAS]
- glutamine catabolic process [IDA, NAS]
- neurotransmitter secretion [TAS]
- protein homotetramerization [IDA]
- small molecule metabolic process [TAS]
- synaptic transmission [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
GLS2
Gene Ontology Biological Process
- cellular amino acid biosynthetic process [TAS]
- cellular amino acid metabolic process [TAS]
- cellular nitrogen compound metabolic process [TAS]
- glutamate secretion [TAS]
- neurotransmitter secretion [TAS]
- reactive oxygen species metabolic process [IMP]
- regulation of apoptotic process [IMP]
- small molecule metabolic process [TAS]
- synaptic transmission [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Cross-Linking-MS (XL-MS)
An interaction is detected between two proteins using chemically reactive or photo-activatable cross-linking reagents that covalently link amino acids in close proximity, followed by mass spectrometry analysis to identify the linked peptides (reviewed in PMID 37406423, 37104977). Experiments may be carried with live cells or cell lysates in which all proteins are expressed at endogenous levels (e.g. PMID 34349018, 35235311) or with recombinant proteins (e.g., PMID 28537071).
Publication
In-Depth In Vivo Crosslinking in Minutes by a Compact, Membrane-Permeable, and Alkynyl-Enrichable Crosslinker.
Chemical crosslinking coupled with mass spectrometry (CXMS) has emerged as a powerful technique to obtain the dynamic conformations and interaction interfaces of protein complexes. Limited by the poor cell membrane permeability, chemical reactivity, and biocompatibility of crosslinkers, in vivo crosslinking to capture the dynamics of protein complexes with finer temporal resolution and higher coverage is attractive but challenging. In this ... [more]
Throughput
- High Throughput
Additional Notes
- High confidence crosslinked peptides were identified with the FDR set to 0.01 at the peptide-spectrum matches (PSM) level and PSMs >= 2.
- MS non-cleavable crosslinker of bis(succinimidyl) with propargyl tag (BSP)
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| GLS2 GLS | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 0.7544 | BioGRID | 3297823 | |
| GLS2 GLS | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 0.9991 | BioGRID | 3054966 |
Curated By
- BioGRID