BAIT

SLC25A18

GC2
solute carrier family 25 (glutamate carrier), member 18
GO Process (0)
GO Function (0)
GO Component (0)
Homo sapiens
PREY

SLC25A22

EIEE3, GC1, NET44
solute carrier family 25 (mitochondrial carrier: glutamate), member 22
GO Process (2)
GO Function (1)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens

Cross-Linking-MS (XL-MS)

An interaction is detected between two proteins using chemically reactive or photo-activatable cross-linking reagents that covalently link amino acids in close proximity, followed by mass spectrometry analysis to identify the linked peptides (reviewed in PMID 37406423, 37104977). Experiments may be carried with live cells or cell lysates in which all proteins are expressed at endogenous levels (e.g. PMID 34349018, 35235311) or with recombinant proteins (e.g., PMID 28537071).

Publication

In-Depth In Vivo Crosslinking in Minutes by a Compact, Membrane-Permeable, and Alkynyl-Enrichable Crosslinker.

Gao H, Zhao L, Zhong B, Zhang B, Gong Z, Zhao B, Liu Y, Zhao Q, Zhang L, Zhang Y

Chemical crosslinking coupled with mass spectrometry (CXMS) has emerged as a powerful technique to obtain the dynamic conformations and interaction interfaces of protein complexes. Limited by the poor cell membrane permeability, chemical reactivity, and biocompatibility of crosslinkers, in vivo crosslinking to capture the dynamics of protein complexes with finer temporal resolution and higher coverage is attractive but challenging. In this ... [more]

Anal Chem May. 31, 2022; 94(21);7551-7558 [Pubmed: 35575683]

Throughput

  • High Throughput

Additional Notes

  • High confidence crosslinked peptides were identified with the FDR set to 0.01 at the peptide-spectrum matches (PSM) level and PSMs >= 2.
  • MS non-cleavable crosslinker of bis(succinimidyl) with propargyl tag (BSP)

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
SLC25A22 SLC25A18
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High0.9999BioGRID
1184950
SLC25A22 SLC25A18
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High0.9999BioGRID
2225774
SLC25A22 SLC25A18
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High0.9999BioGRID
3028616
SLC25A18 SLC25A22
Cross-Linking-MS (XL-MS)
Cross-Linking-MS (XL-MS)

An interaction is detected between two proteins using chemically reactive or photo-activatable cross-linking reagents that covalently link amino acids in close proximity, followed by mass spectrometry analysis to identify the linked peptides (reviewed in PMID 37406423, 37104977). Experiments may be carried with live cells or cell lysates in which all proteins are expressed at endogenous levels (e.g. PMID 34349018, 35235311) or with recombinant proteins (e.g., PMID 28537071).

High-BioGRID
3677558

Curated By

  • BioGRID