ATP1A3
Gene Ontology Biological Process
- cell communication by electrical coupling involved in cardiac conduction [TAS]
- cellular potassium ion homeostasis [IDA]
- cellular response to steroid hormone stimulus [NAS]
- cellular sodium ion homeostasis [IDA]
- ion transmembrane transport [TAS]
- potassium ion import [IDA]
- response to glycoside [NAS]
- sodium ion export from cell [IDA]
- transmembrane transport [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
ATP1A2
Gene Ontology Biological Process
- cardiac muscle contraction [TAS]
- cell communication by electrical coupling involved in cardiac conduction [TAS]
- cellular potassium ion homeostasis [IDA]
- cellular response to steroid hormone stimulus [IDA]
- cellular sodium ion homeostasis [IDA]
- ion transmembrane transport [TAS]
- membrane depolarization during cardiac muscle cell action potential [TAS]
- potassium ion import [IDA]
- potassium ion transport [NAS]
- regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion [TAS]
- regulation of striated muscle contraction [NAS]
- relaxation of cardiac muscle [TAS]
- response to glycoside [IC]
- sodium ion export from cell [IDA]
- sodium ion transport [NAS]
- transmembrane transport [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Cross-Linking-MS (XL-MS)
An interaction is detected between two proteins using chemically reactive or photo-activatable cross-linking reagents that covalently link amino acids in close proximity, followed by mass spectrometry analysis to identify the linked peptides (reviewed in PMID 37406423, 37104977). Experiments may be carried with live cells or cell lysates in which all proteins are expressed at endogenous levels (e.g. PMID 34349018, 35235311) or with recombinant proteins (e.g., PMID 28537071).
Publication
In-Depth In Vivo Crosslinking in Minutes by a Compact, Membrane-Permeable, and Alkynyl-Enrichable Crosslinker.
Chemical crosslinking coupled with mass spectrometry (CXMS) has emerged as a powerful technique to obtain the dynamic conformations and interaction interfaces of protein complexes. Limited by the poor cell membrane permeability, chemical reactivity, and biocompatibility of crosslinkers, in vivo crosslinking to capture the dynamics of protein complexes with finer temporal resolution and higher coverage is attractive but challenging. In this ... [more]
Throughput
- High Throughput
Additional Notes
- High confidence crosslinked peptides were identified with the FDR set to 0.01 at the peptide-spectrum matches (PSM) level and PSMs >= 2.
- MS non-cleavable crosslinker of bis(succinimidyl) with propargyl tag (BSP)
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| ATP1A3 ATP1A2 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 1 | BioGRID | 3108706 | |
| ATP1A2 ATP1A3 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | 0.0101 | BioGRID | 3584373 |
Curated By
- BioGRID