BAIT

FAM134B

JK-1, JK1
family with sequence similarity 134, member B
Autophagy Project
GO Process (1)
GO Function (0)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Homo sapiens
PREY

CKAP4

CLIMP-63, ERGIC-63, p63
cytoskeleton-associated protein 4
GO Process (0)
GO Function (1)
GO Component (3)

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Ubiquitination regulates ER-phagy and remodelling of endoplasmic reticulum.

Gonzalez A, Covarrubias-Pinto A, Bhaskara RM, Glogger M, Kuncha SK, Xavier A, Seemann E, Misra M, Hoffmann ME, Braeuning B, Balakrishnan A, Qualmann B, Doetsch V, Schulman BA, Kessels MM, Huebner CA, Heilemann M, Hummer G, Dikic I

The endoplasmic reticulum (ER) undergoes continuous remodelling via a selective autophagy pathway, known as ER-phagy1. ER-phagy receptors have a central role in this process2, but the regulatory mechanism remains largely unknown. Here we report that ubiquitination of the ER-phagy receptor FAM134B within its reticulon homology domain (RHD) promotes receptor clustering and binding to lipidated LC3B, thereby stimulating ER-phagy. Molecular dynamics (MD) ... [more]

Nature Jun. 01, 2023; 618(7964);394-401 [Pubmed: 37225996]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
CKAP4 FAM134B
Proximity Label-MS
Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

High95BioGRID
2983339

Curated By

  • BioGRID