Publication

MECP2 directly interacts with RNA polymerase II to modulate transcription in human neurons.

Liu Y, Flamier A, Bell GW, Diao AJ, Whitfield TW, Wang HC, Wu Y, Schulte F, Friesen M, Guo R, Mitalipova M, Liu XS, Vos SM, Young RA, Jaenisch R

Mutations in the methyl-DNA-binding protein MECP2 cause the neurodevelopmental disorder Rett syndrome (RTT). How MECP2 contributes to transcriptional regulation in normal and disease states is unresolved; it has been reported to be an activator and a repressor. We describe here the first integrated CUT&Tag, transcriptome, and proteome analyses using human neurons with wild-type (WT) and mutant MECP2 molecules. MECP2 occupies ... [more]

Quantitative Score

• 15.59368747 [Binding Intensity]

Throughput

• High Throughput

Additional Notes

• Within each experiment, reporter intensity values (protein peak areas) are normalized by calculating the median for each sample, equalizing the medians across all samples, and then subtracting the AP-MS results from the negative control (GFP-empty). Hits where all three replicates show a positive value after this subtraction are considered potential MECP2 interactors. The score represents the log2-transformed average of the adjusted binding intensity from three replicates.

Related interactions

Curated By

• BioGRID