An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

The E3 ubiquitin ligase FBXL6 controls the quality of newly synthesized mitochondrial ribosomal proteins.

Lavie J, Lalou C, Mahfouf W, Dupuy JW, Lacaule A, Cywinska AA, Lacombe D, Duchene AM, Raymond AA, Rezvani HR, Ngondo RP, Benard G

In mammals, about 99% of mitochondrial proteins are synthesized in the cytosol as precursors that are subsequently imported into the organelle. The mitochondrial health and functions rely on an accurate quality control of these imported proteins. Here, we show that the E3 ubiquitin ligase F box/leucine-rich-repeat protein 6 (FBXL6) regulates the quality of cytosolically translated mitochondrial proteins. Indeed, we found ... [more]

Cell Rep Jun. 27, 2023; 42(6);112579 [Pubmed: 37267103]

Throughput

High Throughput

Curated By

BioGRID

Interaction Summary

FBXL6

Gene Ontology Biological Process

Gene Ontology Molecular Function

ubiquitin-protein transferase activity [TAS]

CCNK

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA polymerase II carboxy-terminal domain kinase activity [IDA]cyclin-dependent protein serine/threonine kinase activity [IDA]protein binding [IPI]protein kinase binding [IPI]

Gene Ontology Cellular Component

Affinity Capture-MS

Publication

The E3 ubiquitin ligase FBXL6 controls the quality of newly synthesized mitochondrial ribosomal proteins.

Throughput

Curated By

RNA polymerase II carboxy-terminal domain kinase activity [IDA]
cyclin-dependent protein serine/threonine kinase activity [IDA]
protein binding [IPI]
protein kinase binding [IPI]