An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

Analysis of Ku70 S155 Phospho-Specific BioID2 Interactome Identifies Ku Association with TRIP12 in Response to DNA Damage.

Abbasi S, Bayat L, Schild-Poulter C

The Ku heterodimer, composed of subunits Ku70 and Ku80, is known for its essential role in repairing double-stranded DNA breaks via non-homologous end joining (NHEJ). We previously identified Ku70 S155 as a novel phosphorylation site within the von Willebrand A-like (vWA) domain of Ku70 and documented an altered DNA damage response in cells expressing a Ku70 S155D phosphomimetic mutant. Here, ... [more]

Int J Mol Sci Apr. 11, 2023; 24(8); [Pubmed: 37108203]

Throughput

Low Throughput

Additional Notes

BioID

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
EEF2 XRCC6	Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.	Havugimana PC (2012)	High	0.816	BioGRID	750500

Curated By

BioGRID

Interaction Summary

XRCC6

Gene Ontology Biological Process

Gene Ontology Molecular Function

5'-deoxyribose-5-phosphate lyase activity [IMP]DNA binding [NAS]double-stranded DNA binding [TAS]double-stranded telomeric DNA binding [IDA]poly(A) RNA binding [IDA]protein C-terminus binding [IPI]protein binding [IPI]transcription regulatory region DNA binding [IDA]

Gene Ontology Cellular Component

EEF2

Gene Ontology Biological Process

Gene Ontology Molecular Function

poly(A) RNA binding [IDA]protein binding [IPI]protein kinase binding [IPI]translation activator activity [IGI]