An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

HAPSTR1 localizes HUWE1 to the nucleus to limit stress signaling pathways.

Monda JK, Ge X, Hunkeler M, Donovan KA, Ma MW, Jin CY, Leonard M, Fischer ES, Bennett EJ

HUWE1 is a large, enigmatic HECT-domain ubiquitin ligase implicated in the regulation of diverse pathways, including DNA repair, apoptosis, and differentiation. How HUWE1 engages its structurally diverse substrates and how HUWE1 activity is regulated are unknown. Using unbiased quantitative proteomics, we find that HUWE1 targets substrates in a largely cell-type-specific manner. However, we identify C16orf72/HAPSTR1 as a robust HUWE1 substrate ... [more]

Cell Rep May. 30, 2023; 42(5);112496 [Pubmed: 37167062]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
HUWE1 COPS3	Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.	Havugimana PC (2022)	High	-	BioGRID	3452440

Curated By

BioGRID

Interaction Summary

HUWE1

Gene Ontology Biological Process

Gene Ontology Molecular Function

DNA binding [ISS]poly(A) RNA binding [IDA]protein binding [IPI]ubiquitin-protein transferase activity [IDA]

Gene Ontology Cellular Component

COPS3