An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

KCTD13-mediated ubiquitination and degradation of GluN1 regulates excitatory synaptic transmission and seizure susceptibility.

Gu J, Ke P, Guo H, Liu J, Liu Y, Tian X, Huang Z, Xu X, Xu D, Ma Y, Wang X, Xiao F

Temporal lobe epilepsy (TLE) is the most common and severe form of epilepsy in adults; however, its underlying pathomechanisms remain elusive. Dysregulation of ubiquitination is increasingly recognized to contribute to the development and maintenance of epilepsy. Herein, we observed for the first time that potassium channel tetramerization domain containing 13 (KCTD13) protein, a substrate-specific adapter for cullin3-based E3 ubiquitin ligase, ... [more]

Cell Death Differ Jul. 01, 2023; 30(7);1726-1741 [Pubmed: 37142655]

Throughput

High Throughput|Low Throughput

Curated By

BioGRID

Interaction Summary

KCTD13

Gene Ontology Biological Process

Gene Ontology Molecular Function

GTP-Rho binding [ISO]identical protein binding [IPI]protein domain specific binding [ISO]ubiquitin-protein transferase activity [ISO]

Gene Ontology Cellular Component

PI4KA