RPN8
Gene Ontology Biological Process
Gene Ontology Cellular Component
SEM1
Gene Ontology Biological Process
- SAGA complex localization to transcription regulatory region [IMP]
- exocytosis [IGI, IPI]
- filamentous growth [IMP]
- histone deubiquitination [IMP]
- mRNA export from nucleus [IGI, IMP, IPI]
- maintenance of DNA trinucleotide repeats [IMP]
- proteasome assembly [IMP]
- proteasome-mediated ubiquitin-dependent protein catabolic process [IDA, IMP]
- regulation of cell cycle [IMP]
- ubiquitin-dependent protein catabolic process [IMP]
Gene Ontology Cellular Component
Cross-Linking-MS (XL-MS)
An interaction is detected between two proteins using chemically reactive or photo-activatable cross-linking reagents that covalently link amino acids in close proximity, followed by mass spectrometry analysis to identify the linked peptides (reviewed in PMID 37406423, 37104977). Experiments may be carried with live cells or cell lysates in which all proteins are expressed at endogenous levels (e.g. PMID 34349018, 35235311) or with recombinant proteins (e.g., PMID 28537071).
Publication
High-density chemical cross-linking for modeling protein interactions.
Detailed mechanistic understanding of protein complex function is greatly enhanced by insights from its 3-dimensional structure. Traditional methods of protein structure elucidation remain expensive and labor-intensive and require highly purified starting material. Chemical cross-linking coupled with mass spectrometry offers an alternative that has seen increased use, especially in combination with other experimental approaches like cryo-electron microscopy. Here we report advances ... [more]
Throughput
- Low Throughput
Additional Notes
- High confidence interactions were identified using the PIXL (Protein Interactions from Cross-Linking) algorithm and stringently filtered to 1% FDR.
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| SEM1 RPN8 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | Low | - | BioGRID | - | |
| SEM1 RPN8 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | - | |
| SEM1 RPN8 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 10 | BioGRID | 3612918 | |
| SEM1 RPN8 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | Low | - | BioGRID | - | |
| RPN8 SEM1 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -0.7034 | BioGRID | 2018725 |
Curated By
- BioGRID