An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Protein Binder Toolbox for Studies of Solute Carrier Transporters.

Gelova Z, Ingles-Prieto A, Bohstedt T, Frommelt F, Chi G, Chang YN, Garcia J, Wolf G, Azzollini L, Tremolada S, Scacioc A, Hansen JS, Serrano I, Droce A, Bernal JC, Burgess-Brown NA, Carpenter EP, Duerr KL, Kristensen P, Geertsma ER, Stefanic S, Scarabottolo L, Wiedmer T, Puetter V, Sauer DB, Superti-Furga G

Transporters of the solute carrier superfamily (SLCs) are responsible for the transmembrane traffic of the majority of chemical substances in cells and tissues and are therefore of fundamental biological importance. As is often the case with membrane proteins that can be heavily glycosylated, a lack of reliable high-affinity binders hinders their functional analysis. Purifying and reconstituting transmembrane proteins in their ... [more]

J Mol Biol Aug. 15, 2024; 436(16);168665 [Pubmed: 38878854]

Throughput

High Throughput

Additional Notes

The bait protein was tagged with 2xsterp and 1xHA tag (Strep-HA) and overexpressed in Jump in HEK293T cells. Data were scored with SAINT and an interaction was defined as: Saint Score >= 0.95, FC >= 2 and a BFDR =< 0.01.

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
LGALS8 SLC12A6	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Huttlin EL (2015)	High	1	BioGRID	1181912
LGALS8 SLC12A6	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Huttlin EL (2017)	High	1	BioGRID	2219699
LGALS8 SLC12A6	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Huttlin EL (2021)	High	1	BioGRID	3128063

Curated By

BioGRID

Interaction Summary

SLC12A6

Gene Ontology Biological Process

Gene Ontology Molecular Function

potassium ion transmembrane transporter activity [IDA]potassium:chloride symporter activity [IDA]protein kinase binding [IPI]rubidium ion transmembrane transporter activity [IMP]

Gene Ontology Cellular Component

LGALS8