SMURF1
Gene Ontology Biological Process
- BMP signaling pathway [ISO]
- cell differentiation [ISO, ISS]
- negative regulation of ossification [IMP]
- negative regulation of transforming growth factor beta receptor signaling pathway [ISO]
- proteasome-mediated ubiquitin-dependent protein catabolic process [ISO]
- protein export from nucleus [ISO]
- protein localization to cell surface [ISO]
- protein polyubiquitination [ISO]
- protein ubiquitination [IMP, ISO]
- protein ubiquitination involved in ubiquitin-dependent protein catabolic process [IBA, ISO, ISS]
- receptor catabolic process [ISO]
- transforming growth factor beta receptor signaling pathway [IBA]
- ubiquitin-dependent SMAD protein catabolic process [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
GPBAR1
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
The degradation of TGR5 mediated by Smurf1 contributes to diabetic nephropathy.
The multiple roles of TGR5 in the regulation of glucose metabolism, inflammation, and oxidative stress have drawn attention as therapeutic candidates for diabetes-related kidney disease. However, diabetes induces downregulation of renal TGR5 protein expression, and the regulatory mechanisms have not been clarified. Here, we identify that Smurf1, an E3 ubiquitin ligase, is a critical interactor of TGR5 and mediates the ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| GPBAR1 SMURF1 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | Low | - | BioGRID | - | |
| GPBAR1 SMURF1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - |
Curated By
- BioGRID