BAIT

SIGMAR1

AL024364, Oprs1, sigma1R, RP23-167I12.6
sigma non-opioid intracellular receptor 1
GO Process (3)
GO Function (3)
GO Component (1)
Mus musculus
PREY

RAC1

AL023026, D5Ertd559e
RAS-related C3 botulinum substrate 1
GO Process (51)
GO Function (10)
GO Component (16)

Gene Ontology Biological Process

Mus musculus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Knockdown of interleukin-10 induces the redistribution of sigma1-receptor and increases the glutamate-dependent NADPH-oxidase activity in mouse brain neurons.

Koriauli S, Natsvlishvili N, Barbakadze T, Mikeladze D

In the central nervous system, interleukin-10 (IL-10) provides trophic and survival effects directly on neurons, modulates neurite plasticity, and has a pivotal importance in the neuronal regeneration in neurodegenerative and neuroinflammatory conditions. This cytokine is primarily produced by glial cells and has beneficial effects on the neuronal viability. However, the mechanisms of IL-10-elicited neuroprotection are not clear.Membrane preparations, isolated from ... [more]

Biol Res Oct. 09, 2015; 48();55 [Pubmed: 26453192]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
RAC1 SIGMAR1
Co-localization
Co-localization

Interaction inferred from two proteins that co-localize in the cell by indirect immunofluorescence only when in addition, if one gene is deleted, the other protein becomes mis-localized. Also includes co-dependent association of proteins with promoter DNA in chromatin immunoprecipitation experiments.

Low-BioGRID
2656284

Curated By

  • BioGRID