BAIT

SIGMAR1

AL024364, Oprs1, sigma1R, RP23-167I12.6
sigma non-opioid intracellular receptor 1
GO Process (3)
GO Function (3)
GO Component (1)
Mus musculus
PREY

HSPA5

AL022860, AU019543, Bip, D2Wsu141e, D2Wsu17e, Grp78, Hsce70, SEZ-7, Sez7, baffled, mBiP, RP23-446N16.1
heat shock protein 5
GO Process (15)
GO Function (8)
GO Component (17)
Mus musculus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Knockdown of interleukin-10 induces the redistribution of sigma1-receptor and increases the glutamate-dependent NADPH-oxidase activity in mouse brain neurons.

Koriauli S, Natsvlishvili N, Barbakadze T, Mikeladze D

In the central nervous system, interleukin-10 (IL-10) provides trophic and survival effects directly on neurons, modulates neurite plasticity, and has a pivotal importance in the neuronal regeneration in neurodegenerative and neuroinflammatory conditions. This cytokine is primarily produced by glial cells and has beneficial effects on the neuronal viability. However, the mechanisms of IL-10-elicited neuroprotection are not clear.Membrane preparations, isolated from ... [more]

Biol Res Oct. 09, 2015; 48();55 [Pubmed: 26453192]

Throughput

  • Low Throughput

Curated By

  • BioGRID