An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Dependency of NELF-E-SLUG-KAT2B epigenetic axis in breast cancer carcinogenesis.

Zhang J, Hu Z, Chung HH, Tian Y, Lau KW, Ser Z, Lim YT, Sobota RM, Leong HF, Chen BJ, Yeo CJ, Tan SYX, Kang J, Tan DEK, Sou IF, McClurg UL, Lakshmanan M, Vaiyapuri TS, Raju A, Wong ESM, Tergaonkar V, Rajarethinam R, Pathak E, Tam WL, Tan EY, Tee WW

Cancer cells undergo transcriptional reprogramming to drive tumor progression and metastasis. Using cancer cell lines and patient-derived tumor organoids, we demonstrate that loss of the negative elongation factor (NELF) complex inhibits breast cancer development through downregulating epithelial-mesenchymal transition (EMT) and stemness-associated genes. Quantitative multiplexed Rapid Immunoprecipitation Mass spectrometry of Endogenous proteins (qPLEX-RIME) further reveals a significant rewiring of NELF-E-associated chromatin ... [more]

Nat Commun Apr. 28, 2023; 14(1);2439 [Pubmed: 37117180]

Throughput

High Throughput

Additional Notes

Affinity capture MS was carried out to identify high confidence NELF-E protein interactors

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
YWHAG NELFE	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Segal D (2023)	High	-	BioGRID	3532210

Curated By

BioGRID

Interaction Summary

NELFE

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA binding [NAS]poly(A) RNA binding [IDA]protein binding [IPI]

Gene Ontology Cellular Component

YWHAG

Gene Ontology Biological Process

Gene Ontology Molecular Function

insulin-like growth factor receptor binding [IPI]poly(A) RNA binding [IDA]protein binding [IPI]protein kinase C binding [IPI]protein kinase C inhibitor activity [NAS]