BAIT

NELFE

D6S45, NELF-E, RD, RDBP, RDP, DADB-122G4.4
negative elongation factor complex member E
Homo sapiens
PREY

YWHAQ

14-3-3, 1C5, HS1
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta
Alzheimer's Disease Project
Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Dependency of NELF-E-SLUG-KAT2B epigenetic axis in breast cancer carcinogenesis.

Zhang J, Hu Z, Chung HH, Tian Y, Lau KW, Ser Z, Lim YT, Sobota RM, Leong HF, Chen BJ, Yeo CJ, Tan SYX, Kang J, Tan DEK, Sou IF, McClurg UL, Lakshmanan M, Vaiyapuri TS, Raju A, Wong ESM, Tergaonkar V, Rajarethinam R, Pathak E, Tam WL, Tan EY, Tee WW

Cancer cells undergo transcriptional reprogramming to drive tumor progression and metastasis. Using cancer cell lines and patient-derived tumor organoids, we demonstrate that loss of the negative elongation factor (NELF) complex inhibits breast cancer development through downregulating epithelial-mesenchymal transition (EMT) and stemness-associated genes. Quantitative multiplexed Rapid Immunoprecipitation Mass spectrometry of Endogenous proteins (qPLEX-RIME) further reveals a significant rewiring of NELF-E-associated chromatin ... [more]

Nat Commun Apr. 28, 2023; 14(1);2439 [Pubmed: 37117180]

Throughput

  • High Throughput

Additional Notes

  • Affinity capture MS was carried out to identify high confidence NELF-E protein interactors

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
YWHAQ NELFE
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High-BioGRID
3533564

Curated By

  • BioGRID