DSP
Gene Ontology Biological Process
- adherens junction organization [IMP]
- bundle of His cell to Purkinje myocyte communication [ISO]
- desmosome organization [IMP]
- intermediate filament cytoskeleton organization [IMP]
- intermediate filament organization [IMP]
- keratinocyte differentiation [ISO]
- peptide cross-linking [ISO]
- protein localization to adherens junction [IMP]
- regulation of heart rate by cardiac conduction [ISO]
- single organismal cell-cell adhesion [IMP]
- skin development [IMP]
- ventricular cardiac muscle cell action potential [ISO]
- ventricular compact myocardium morphogenesis [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
DSP
Gene Ontology Biological Process
- adherens junction organization [IMP]
- bundle of His cell to Purkinje myocyte communication [ISO]
- desmosome organization [IMP]
- intermediate filament cytoskeleton organization [IMP]
- intermediate filament organization [IMP]
- keratinocyte differentiation [ISO]
- peptide cross-linking [ISO]
- protein localization to adherens junction [IMP]
- regulation of heart rate by cardiac conduction [ISO]
- single organismal cell-cell adhesion [IMP]
- skin development [IMP]
- ventricular cardiac muscle cell action potential [ISO]
- ventricular compact myocardium morphogenesis [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Cross-Linking-MS (XL-MS)
An interaction is detected between two proteins using chemically reactive or photo-activatable cross-linking reagents that covalently link amino acids in close proximity, followed by mass spectrometry analysis to identify the linked peptides (reviewed in PMID 37406423, 37104977). Experiments may be carried with live cells or cell lysates in which all proteins are expressed at endogenous levels (e.g. PMID 34349018, 35235311) or with recombinant proteins (e.g., PMID 28537071).
Publication
Chemical Crosslinking Mass Spectrometry Analysis of Protein Conformations and Supercomplexes in Heart Tissue.
While modern structural biology technologies have greatly expanded the size and type of protein complexes that can now be studied, the ability to derive large-scale structural information on proteins and complexes as they exist within tissues is practically nonexistent. Here, we demonstrate the application of crosslinking mass spectrometry to identify protein structural features and interactions in tissue samples, providing systems ... [more]
Throughput
- High Throughput
Additional Notes
- High confidence protein interaction identified through a combination of MS3 search results (Comet) from cross-linked peptide pairs enriched from two samples each. The results (2663 XL pairs) were filtered to a maximum evalue of 0.2 resulting in an estimated FDR at the PSM level of 0.007642.
Curated By
- BioGRID